e. coli bl21 (de3) host strain (Thermo Fisher)
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e. coli bl21 (de3) host strain
E. Coli Bl21 (De3) Host Strain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+bl21+(de3)+host+strain/pm39455289-169-13-27
Average 90 stars, based on 1 article reviews
E. Coli Bl21 (De3) Host Strain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+bl21+(de3)+host+strain/pm39455289-169-13-27
Average 90 stars, based on 1 article reviews
e. coli bl21 (de3) host strain - by Bioz Stars,
2026-11
90/100 stars
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other:Article Title: ER-PM Junctions on GABAergic Interneurons Are Organized by Neuregulin 2/VAP Interactions and Regulated by NMDA Receptors. Article Snippet: Expression of GST and GST-NRG2_CD bait proteins in the E. Coli host strain BL21(DE3) ( Article Title: Rational and Semirational Approaches for Engineering Salicylate Production in Escherichia coli . Article Snippet: Salicylate plays a pivotal role as a pharmaceutical intermediate in drugs, such as aspirin and lamivudine.. The low catalytic efficiency of key enzymes and the inherent toxicity of salicylates to cells pose significant challenges to large-scale microbial production.. In this study, we introduced the salicylate synthase Irp9 into an L-phenylalanine-producing Escherichia coli, constructing the shortest salicylate biosynthetic pathway. Article Title: Strenuous expression of Porcine epidemic diarrhea virus ORF3 protein suggests role in pathogenesis Article Snippet: Materials and methods Prokaryotic cell culture For plasmids replication experiments, Article Title: ER-PM Junctions on GABAergic Interneurons Are Organized by Neuregulin 2/VAP Interactions and Regulated by NMDA Receptors Article Snippet: Expression of GST and GST-NRG2_CD bait proteins in the E. Coli host strain BL21(DE3) ( Article Title: Strenuous expression of porcine epidemic diarrhea virus ORF3 protein suggests host resistance. Article Snippet: Porcine epidemic diarrhea virus is attenuated upon adaptation to cell culture.. Exclusively genomic mutations have been traced to the ORF3 gene of the laboratory strains.. Previous attempts to express the protein were unsuccessful. Cell Culture:Article Title: ErpY-LemA: A breakthrough in human leptospirosis diagnosis. Article Snippet: Leptospirosis remains a significant public health concern worldwide, particularly in tropical regions, where its diagnosis is often challenging due to nonspecific symptoms and limitations of current diagnostic methods.. In this study, we evaluated the diagnostic potential of the recombinant chimera ErpY-LemA constructed from two Leptospira proteins with known immunogenic properties.. The chimera was successfully expressed in Escherichia coli and demonstrated strong antigenicity, effectively recognizing sera from hamsters infected with diverse Leptospira serovars, indicating its non-serovar-specific nature (P < 0.001). Amplification:Article Title: Chimeric Article Snippet: .. After amplification by PCR, the amplicon (1,122-nt) was cloned into a pET32a expression plasmid (FIG. 1), confirmed by sequencing (Genewiz Inc., Suzhou, China), and subsequently used to transform the Polymerase Chain Reaction:Article Title: Chimeric Article Snippet: .. After amplification by PCR, the amplicon (1,122-nt) was cloned into a pET32a expression plasmid (FIG. 1), confirmed by sequencing (Genewiz Inc., Suzhou, China), and subsequently used to transform the Clone Assay:Article Title: Chimeric Article Snippet: .. After amplification by PCR, the amplicon (1,122-nt) was cloned into a pET32a expression plasmid (FIG. 1), confirmed by sequencing (Genewiz Inc., Suzhou, China), and subsequently used to transform the Expressing:Article Title: Chimeric Article Snippet: .. After amplification by PCR, the amplicon (1,122-nt) was cloned into a pET32a expression plasmid (FIG. 1), confirmed by sequencing (Genewiz Inc., Suzhou, China), and subsequently used to transform the Article Title: Bone Regeneration in Rat Cranium Critical-Size Defects Induced by Cementum Protein 1 (CEMP1) Article Snippet: Briefly, recombinant clones were constructed with a pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA, USA) for directional cloning of a blunt-end PCR CEMP1 product. .. The plasmid was introduced into Plasmid Preparation:Article Title: Chimeric Article Snippet: .. After amplification by PCR, the amplicon (1,122-nt) was cloned into a pET32a expression plasmid (FIG. 1), confirmed by sequencing (Genewiz Inc., Suzhou, China), and subsequently used to transform the Article Title: Bone Regeneration in Rat Cranium Critical-Size Defects Induced by Cementum Protein 1 (CEMP1) Article Snippet: Briefly, recombinant clones were constructed with a pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA, USA) for directional cloning of a blunt-end PCR CEMP1 product. .. The plasmid was introduced into Sequencing:Article Title: Chimeric Article Snippet: .. After amplification by PCR, the amplicon (1,122-nt) was cloned into a pET32a expression plasmid (FIG. 1), confirmed by sequencing (Genewiz Inc., Suzhou, China), and subsequently used to transform the |